Biochemical Effects of Oral Contraceptive Pill On Total Serum Protein, Hemoglobin and Antioxidants Capacity among Females Athletes

Biochemical Effects of Oral Contraceptive Pill


  • Alamgir Khan Department of Sports Sciences & Physical Education, University of the Punjab, Lahore, Pakistan
  • Muhammad Jamil Punajb Highway Patrolling Police Lahore, Pakistan
  • Muhammad Zafar Iqbal Butt Department of Sports Sciences & Physical Education, University of the Punjab, Lahore, Pakistan
  • Ausaf Chaudhary International Islamic University, Islamabad
  • Aftab Ahmad Jan Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, KPK, Pakistan
  • Zeliha Selamoglu Department of Medical Biology, Faculty of Medicine, Nigde Ömer Halisdemir University, Nigde, Turkey
  • Elifsena Canan Alp Elifsena Canan Alp Department of Obstetrics and Gynecology, Faculty of Medicine, Selcuklu, Necmettin Erbakan University Meram, Konya, Turkey



Contraceptive Pills, Total Serum Protein, Hemoglobin, Antioxidants Capacity, Female.


Total serum protein, hemoglobin and antioxidant system are essential to the body's physiological activities. Oral contraceptive pills influence the level of proteins of both types present in the blood; thus, it is found to change among pregnant women. Objectives: To scrutinize  oral contraceptive pills' effects (OCP) on total serum protein, hemoglobin and antioxidant capacity among females. Methods: The participants were divided into the experimental group (EG-60) and control group (CG,-24). 5 ml of blood was collected from each subject and marked with a different identification code. The collected blood samples were processed through laboratory protocols, and thus the targeted tests were performed to achieve the desired results. The results of tests were processed through a statistical package for social sciences (SPSS, version-26), and thus appropriate statistical tools were applied for analysis. Results: Total number of subjects in both groups were 84. Mean of CG in term of hemoglobin was 13.91 ±.92 and Mean of EG in term of hemoglobin was 14.18 ±.84. Mean of CG  in term of total serum protein was 6.75 ±.48 and  Mean of EG in term of total serum protein was 6.62 ±..31. Conclusion: Based on the analysis, the researcher concluded that contraceptive pills significantly affect total serum protein, hemoglobin and antioxidant capacity among females.


Spencer AL, Bonnema R, McNamara MC. Helping women choose appropriate hormonal contraception: update on risks, benefits, and indications. The American journal of medicine. 2009 Jun; 122(6): 497-506. doi: 10.1016/j.amjmed.2009.01.016

Lewis NA, Howatson G, Morton K, Hill J, Pedlar CR. Alterations in redox homeostasis in the elite endurance athlete. Sports medicine. 2015 Mar; 45(3): 379-409. doi: 10.1007/s40279-014-0276-5

Powers SK, Ji LL, Leeuwenburgh CH. Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review. Medicine and science in sports and exercise. 1999 Jul; 31(7): 987-97. doi: 10.1097/00005768-199907000-00011

Kerksick CM and Zuhl M. Mechanisms of oxidative damage and their impact on contracting muscle. Antioxidants in sport nutrition. 2015 Jun: 1-6.

Lichtenberg D and Pinchuk I. Oxidative stress, the term and the concept. Biochemical and Biophysical Research Communications. 2015 Jun; 461(3): 441-4. doi: 10.1016/j.bbrc.2015.04.062

Chen JT, Kotani K. Oral contraceptive therapy increases oxidative stress in pre-menopausal women. International journal of preventive medicine. 2012 Dec; 3(12): 893-896.

Hernández I, Delgado JL, Díaz J, Quesada T, Teruel MJ, Llanos MC, et al. 17β-Estradiol prevents oxidative stress and decreases blood pressure in ovariectomized rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2000 Nov; 279(5): 599-605. doi: 10.1152/ajpregu.2000.279.5.R1599

Misra R, Mangi S, Joshi S, Mittal S, Gupta SK, Pandey RM. LycoRed as an alternative to hormone replacement therapy in lowering serum lipids and oxidative stress markers: a randomized controlled clinical trial. Journal of Obstetrics and Gynaecology Research. 2006 Jun; 32(3): 299-304. doi: 10.1111/j.1447-0756.2006.00410.x

Wu G. Functional amino acids in growth, reproduction, and health. Advances in nutrition. 2010 Nov; 1(1): 31-7. doi: 10.3945/an.110.1008

Wu G. Dietary requirements of synthesizable amino acids by animals: a paradigm shift in protein nutrition. Journal of animal science and biotechnology. 2014 Dec; 5(1): 1-2. doi: 10.1186/2049-1891-5-34

O'Connell T, Horita TJ, Kasravi B. Understanding and interpreting the serum protein electrophoresis. American family physician. 2005 Jan; 71(1): 105-12.

Crisman MV, Scarratt WK, Zimmerman KL. Blood proteins and inflammation in the horse. Veterinary Clinics of North America: Equine Practice. 2008 Aug; 24(2): 285-97. doi: 10.1186/s13028-016-0233-4

Bobbo T, Fiore E, Gianesella M, Morgante M, Gallo L, Ruegg PL, Bittante G, Cecchinato A. Variation in blood serum proteins and association with somatic cell count in dairy cattle from multi-breed herds. Animal. 2017 Dec; 11(12): 2309-19. doi: 10.1017/S1751731117001227

Sahu S, Das BK, Mishra BK, Pradhan J, Sarangi N. Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Journal of Applied Ichthyology. 2007 Feb; 23(1): 80-6. doi: 10.1111/j.1439-0426.2006.00785.x

Gleichmann W, Bachmann GW, Dengler HJ, Dudeck J. Effects of hormonal contraceptives and pregnancy on serum protein pattern. European Journal of Clinical Pharmacology. 1973 Apr; 5(4): 218-25. doi: 10.1007/BF00567007

Glenn A and Armstrong CE. Physiology of red and white blood cells. Anaesthesia & Intensive Care Medicine. 2019 Mar; 20(3): 170-4. doi: 10.1016/j.mpaic.2021.10.019

Cappellini MD and Motta I. Anemia in clinical practice—definition and classification: does hemoglobin change with aging?. Seminars in hematology 2015 Oct; 52(4): 261-9. doi: 10.1053/j.seminhematol.2015.07.006

Beutler E and Waalen J. The definition of anemia: what is the lower limit of normal of the blood hemoglobin concentration?. Blood. 2006 Mar; 107(5): 1747-50. doi: 10.1182/blood-2005-07-3046

Norouzi V, Seifi M, Fallah S, Korani M, Samadikuchaksaraei A. Effect of oral contraceptive therapy on homocysteine and C-reactive protein levels in women: an observational study. Anatolian Journal of Cardiology/Anadolu Kardiyoloji Dergisi. 2011 Dec; 11(8): 698-702. doi: 10.5152/akd.2011.191

Adejumo EN, Adediji IO, Akinmulero AO. Effect of Hormonal Contraceptives on the Total Antioxidants Status of Women from Isolo, Lagos State, Nigeria. Journal of Biosciences and Medicines. 2016; 4(01): 107-11. doi: 10.4236/jbm.2016.41013

Manzoor KH, Alamgir KH, İqbal Z, Samiullah KH, Jamil M, Özdemir B, et al. Oxidative Stress and Menstrual Complications Caused by Vaccination of COVID-19 Among Females Athletes. Cumhuriyet Medical Journal. 2022 Mar; 44(1): 38-43. doi: 10.7197/cmj.1035772

Anderson FD, Gibbons W, Portman D. Long-term safety of an extended-cycle oral contraceptive (Seasonale): a 2-year multicenter open-label extension trial. American journal of obstetrics and gynecology. 2006 Jul; 195(1): 92-6. doi: 10.1016/j.ajog.2005.12.045

Nappi RE, Kaunitz AM, Bitzer J. Extended regimen combined oral contraception: A review of evolving concepts and acceptance by women and clinicians. The European Journal of Contraception & Reproductive Health Care. 2016 Mar 3; 21(2): 106-15. doi: 10.3109/13625187.2015.1107894

A Garside D, Gebril A, Nimmo N, Alsaadi M, B Mullen A, A Ferro V. An update on developments in female hormonal contraception. Current Women's Health Reviews. 2012 Nov; 8(4): 276-88



DOI: 10.54393/pjhs.v3i06.341
Published: 2022-11-30

How to Cite

Khan, A. ., Jamil, M. ., Zafar Iqbal Butt, M. ., Chaudhary, A. ., Ahmad Jan, A. ., Selamoglu, Z. ., & Canan Alp, E. . (2022). Biochemical Effects of Oral Contraceptive Pill On Total Serum Protein, Hemoglobin and Antioxidants Capacity among Females Athletes : Biochemical Effects of Oral Contraceptive Pill. Pakistan Journal of Health Sciences, 3(06), 216–219.



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