Variation of Epithelial and Nuclei Morphohistology in Oral Submucous Fibrosis Patients: Epithelial and Nuclei Morphohistology in Oral Submucous Fibrosis Patients

Rida Rubab Ahmed; Maryam Faiz Qureshi; Sibgha Bashir; Zen Ul Abideen; Adeel Zafar; Rizwan Hafeez; Mazhar Ul Haque

doi:10.54393/pjhs.v6i1.1659

Authors

Rida Rubab Ahmed Department of Anatomy, Shahida Islam Medical and Dental College, Lodhran, Pakistan
Maryam Faiz Qureshi Department of Anatomy, Western University of Health Sciences, California
Sibgha Bashir Department of Pathology, Shahida Islam Medical and Dental College, Lodhran, Pakistan
Zen Ul Abideen Department of Anatomy, Combined Military Hospital, Institute of Medical Sciences, Bahawalpur, Pakistan
Adeel Zafar Department of Pathology, Shahida Islam Medical and Dental College, Lodhran, Pakistan
Rizwan Hafeez Department of Medicine, Shahida Islam Medical and Dental College, Lodhran, Pakistan
Mazhar Ul Haque Department of Anatomy, Shahida Islam Medical and Dental College, Lodhran, Pakistan

DOI:

https://doi.org/10.54393/pjhs.v6i1.1659

Keywords:

Oral Submucous Fibrosis, Histological Changes, Epithelial Textural Properties, Nuclear Morphology, Premalignancy

Abstract

In the background of oral submucous fibrosis, oral cancer is the most commonly present malignant transformation in South Asian regions. Alteration of tissue architecture contributes significantly to determine the exact pathological state of the disease. Objective: To evaluate histological changes in structure of oral submucous fibrosis patients in context of textural properties of epithelium and morphology of nuclei. Methods: A total of 50 subjects were inducted in this cross-sectional study performed at dentistry department of Jinnah Postgraduate Medical Center (JPMC) in Karachi. Punch biopsy samples were taken from buccal mucosa followed by preparation of tissue blocks and slide preparation to analyze histology of premalignancy and record abnormal features present in normal oral tissue. Results were analyzed through SPSS version 23.0 and p value of ≤0.05 was considered as statistically significant. Results: 37 (74%) subjects had prominent Oral Submucous Fibrosis histologically out of which abnormal epithelial parameters such as stratified squamous, hyperplastic, keratotic, and neoplastic were noted in 5 (10%), 31 (62%), 10 (2%), 0 (0%) subject respectively. p value 0.041 was calculated using Chi square analysis for qualitative data which depicted relevance of hyperplastic epithelium in Oral Submucous Fibrosis. 29 (87.9%) subjects showed pleomorphism in nucleus and 8 (47.1%) showed normal/round nucleus in Oral Submucous Fibrosis subjects with a p value of 0.003. Conclusions: Histological analysis which is not routinely performed in oral submucous fibrosis patients could visualize accurate extent of disease through structural variability of epithelium and nucleus.

Author Biography

Zen Ul Abideen, Department of Anatomy, Combined Military Hospital, Institute of Medical Sciences, Bahawalpur, Pakistan

Department of Anatomy, Assistant Professor

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