Synthesis, Characterization and Drug Release Study of Novel Guided Tissue Regeneration Membranes Containing Drug Loaded Chitosan Nanoparticles: Tissue Regeneration Membranes Containing Chitosan Nanoparticles

Muhammad Asad Ghaffar; Maleeha Nayyer; Muhammad Kaleem; Muhammad Azhar; Asma Tufail Shah; Safi Ullah Khan

doi:10.54393/pjhs.v5i03.795

Authors

Muhammad Asad Ghaffar Department of Dental Materials, Army Medical College/ National University of Medical Sciences, Islamabad, Pakistan
Maleeha Nayyer Department of Dental Materials, Army Medical College/ National University of Medical Sciences, Islamabad, Pakistan
Muhammad Kaleem 1Department of Dental Materials, Army Medical College/ National University of Medical Sciences, Islamabad, Pakistan
Muhammad Azhar Department of Oral Biology, Army Medical College/ National University of Medical Sciences, Islamabad, Pakistan
Asma Tufail Shah Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Lahore, Pakistan
Safi Ullah Khan Department of Oral Biology, Multan Medical and Dental College, Multan, Pakistan

DOI:

https://doi.org/10.54393/pjhs.v5i03.795

Keywords:

Chitosan Nanoparticles, GTR Membrane, Drug Loaded Nanoparticles, Ciprofloxacin

Abstract

Periodontitis is an inflammatory disease which can cause the destruction of the supporting tissues of the tooth leading to tooth loss. The guided tissue regeneration is considered as a gold standard for its treatment but the re-infection of surgical site limits its overall success. Objective: To synthesize novel monolayer guided tissue regeneration (GTR) membrane containing drug loaded chitosan nanoparticles and to evaluate the drug release from the synthesized GTR membranes. Methods: The chitosan nanoparticles containing ciprofloxacin were synthesized by the ionotropic gelation method and these synthesized nanoparticles were added into chitosan GTR membrane fabricated by the freeze gelation method. For comparison GTR membrane was prepared as a control by freeze gelation method in which the drug was added directly. The prepared membranes were characterized by the SEM and FTIR. The drug release was measured from the membrane samples in the phosphate buffer saline (PBS) at 1, 3, 5, 7 and 9 days. Results: The GTR membrane containing the ciprofloxacin loaded chitosan nanoparticles showed fast drug release as compared to the membrane in which the ciprofloxacin was added directly. Conclusions: The inclusion of antibiotic loaded chitosan nanoparticles can increase the drug release from GTR membrane.

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