Frequency of Human Immunodeficiency Virus, Hepatitis B, and Hepatitis C Among Antenatal Women at a Tertiary Care Hospital
HIV, HBV, and HCV Prevalence in Antenatal Women
DOI:
https://doi.org/10.54393/pjhs.v7i6.3017Keywords:
Hepatitis B, Hepatitis C, Human Immunodeficiency Virus, Antenatal CareAbstract
Human Immunodeficiency Virus (HIV), Hepatitis B (HBV), and Hepatitis C (HCV) are major blood-borne infections. In Pakistan, the prevalence of HCV is predicted to range from 8% to 15%, while the combined frequency of HBV and HCV affects 7.6% of the population. Prenatal HIV screening is recommended to prevent mother-to-child transmission. Objectives: This study aimed to determine the frequency of HIV, HBV, and HCV among antenatal patients at a tertiary healthcare facility. Methods: A descriptive cross-sectional study was carried out at the Civil Hospital Karachi Obstetrics and Gynecology unit 3 over 6 months, from October 7, 2022, to April 8, 2023. Pregnant women aged between 15-49 years, with a gestational age >20 weeks, presenting for routine antenatal care were enrolled using non probability consecutive sampling. All participants were screened for HIV, HBV, and HCV. Data were analyzed using SPSS version 25.0. Results: Among 187 women, the mean age was 32.29 ± 3.91 years, and the mean gestational age was 22.87 ± 1.34 weeks. The prevalence rates of HIV, HBV, and HCV were 13 (7%), 23 (12.3%), and 43 (23%), respectively. Conclusions: Among antenatal patients, the prevalence of HIV, HBV, and HCV was 13 (7%), 23 (12.3%), and 43 (23%), respectively. This underscores the need for regular screening during pregnancy. Regular screening during pregnancy is mandatory to prevent these infections from being transferred from the mother to the child.
References
Dagnew M, Million Y, Gizachew M, Eshetie S, Yitayew G, Asrade L et al. Hepatitis B and C Virus Infection and Associated Factors Among Pregnant Women Attending Antenatal Care in Hospitals in the Amhara National Regional State, Ethiopia. International Journal of Microbiology. 2020; 2020: 8848561. doi: 10.1155/2020/8848561.
Wu S, Wang J, Guo Q, Lan H, Sun Y, Ren M et al. Prevalence of Human Immunodeficiency Virus, Syphilis, and Hepatitis B and C Virus Infections in Pregnant Women: A Systematic Review and Meta-Analysis. Clinical Microbiology and Infection. 2023; 29(8): 1000-1007. doi: 10.1016/j.cmi.2023.03.002.
Beard N and Hill A. Combined “Test and Treat” Campaigns for Human Immunodeficiency Virus, Hepatitis B, and Hepatitis C: A Systematic Review to Provide Evidence to Support World Health Organization Treatment Guidelines. Open Forum Infectious Diseases. 2024; 11(2):. 666. doi: 10.1093/ofid/ofad666
Arbune M, Iancu AV, Padurariu-Covit MD, Plesea-Condratovici A, Arbune AA, Plesea-Condratovici C. Triple Burden of HIV, HBV, and HDV in Adults with Childhood Parenterally Acquired Infections: A Romanian Single-Center Study. Pathogens. 2025; 14(12): 1261. doi: 10.3390/pathogens14121261.
World Health Organization. Global Hepatitis Report 2024: Action for Access in Low- And Middle-Income Countries. Geneva: WHO; 2024.
Sticher JS, Csermak KR, Otsyula N, Turkson M, Aubrun E, Oshagbemi OA. Prevalence of Viral Hepatitis in Sub-Saharan Africa Among the General Population: An Umbrella Review of Systematic Reviews and Meta-Analyses. British Medical Journal Open. 2025; 15(9) :101029. doi: 10.1136/bmjopen-2025-101029.
Hassan-Kadle MA, Keles E, Nor MA, Hassan MA, Karaketir S, Hussein AI et al. Seroprevalence of Hepatitis B, Hepatitis C, and HIV in Pregnant Women Attending a Tertiary Care Hospital in Mogadishu, Somalia, 2017–2021. BioMed Central Infectious Diseases. 2025; 25(1): 885. doi: 10.1186/s12879-025-11268-9.
Qureshi HZ, Khan B, Iftikhar A, Imran H, Iqbal MA. Prevalence and Risk Factors of Hepatitis B and C in Pregnant Women at Bakhtawar Amin Trust Hospital, Multan, Pakistan. Life Science. 2025; 6(3): 387–393. doi: 10.37185/LnS.1.1.569.
Ehsan A, Robert H, Nabi I, Abbas M. Prevalence of Hepatitis B and C in Female Population Reporting for Antenatal Screening at Combined Military Hospital, Hyderabad, Sindh, Pakistan. Pakistan Armed Forces Medical Journal. 2020; 70(2): 385–389.
World Health Organization. Prevalence of hepatitis and HIV in Pakistan. East Mediterr Health Journal. 2024; 30(10). doi: 10.26719/2024.30.10.689.
Tanveer Z, Ahmad I, Javed MS, Malik SA, Kakar NH, Ahmad M et al. Screening for Hepatitis B and C Viral Infections Among Pregnant Women Attending Bolan Medical Complex Hospital and Sandeman Provincial Civil Hospital, Quetta, Pakistan. The Professional Medical Journal. 2020; 27(7): 1328–1334. doi: 10.29309/TPMJ/2020.27.08.3129.
Rios J, Alpert L, Mehra S, Schmidt N, Kushner T. Overview of Hepatitis C in Pregnancy: Screening, Management, and Treatment. Journal of the Pediatric Infectious Diseases Society. 2024; 13(5): 171–178. doi: 10.1093/jpids/piae070.
Society for Maternal-Fetal Medicine. SMFM consult series #69: Hepatitis B in Pregnancy: Updated Guidelines. American Journal of Obstetrics and Gynecology. 2024; 230(4): 2–11. doi: 10.1016/j.ajog.2023.12.023.
Duncan D, Duncan J, Kramer B, Nilsson AY, Haile B, Butcher A et al. An HIV Diagnostic Testing Algorithm Using the Cobas HIV-1/HIV-2 Qualitative Assay for HIV Type Differentiation and Confirmation. Journal of Clinical Microbiology. 2021; 59(7): 0303020. doi: 10.1128/JCM.03030-20.
Fleșeriu T, Meliț LE, Mărginean CO, Pop AV, Văsieșiu AM. Maternal HIV Infection and Antiretroviral Therapy in Pregnancy: Implications for Vertical Transmission and Outcomes. Pathogens. 2025; 14(8): 818. doi: 10.3390/pathogens14080818.
Roble AK, Roba KT, Mengistie B, Abdurke Kure M. Seroprevalence of Hepatitis B Virus and Associated Factors Among Pregnant Women Attending Antenatal Care in Public Health Facilities in Jigjiga Town, Eastern Ethiopia. International Journal of Women's Health. 2021; 12: 1299–1310. doi: 10.2147/IJWH.S276526.
Ikeako L, Ezegwui H, Ajah L, Dim C, Okeke T. Seroprevalence of Human Immunodeficiency Virus, Hepatitis B, Hepatitis C, Syphilis, and Co-Infections Among Antenatal Women in a Tertiary Institution in South East, Nigeria. Annals of Medical and Health Sciences Research. 2014; 4(6): 954–958. doi: 10.4103/2141-9248.144925.
Vueba AN, Almendra R, Santana P, Faria C, do Céu Sousa M. Prevalence of HIV and Hepatitis B Virus Among Pregnant Women in Luanda (Angola): Geospatial Distribution and Association with Socio-Demographic and Clinical-Obstetric Determinants. Virology Journal. 2021; 18(1): 1–9. doi: 10.1186/s12985-021-01698-7.
Amsalu A, Ferede G, Eshetie S, Tadewos A, Assegu D. Prevalence, Infectivity, and Associated Risk Factors of Hepatitis B Virus Among Pregnant Women in Yirgalem Hospital, Ethiopia: Implication of Screening to Control Mother-To-Child Transmission. Journal of Pregnancy. 2018: 8435910. doi: 10.1155/2018/8435910.
Nakazwe C, Michelo C, Sandøy IF, Fylkesnes K. Contrasting HIV Prevalence Trends Among Young Women and Men in Zambia in the Past 12 Years: Data from Demographic and Health Surveys 2002–2014. BioMed Central Infectious Diseases. 2019; 19(1): 432. doi: 10.1186/s12879-019-4059-3.
Pour M, James L, Singh K, Mampunza S, Baer F, Scott J et al. Increased HIV in Greater Kinshasa Urban Health Zones, Democratic Republic of Congo (2017–2018). AIDS Research and Therapy. 2020; 17(1): 67. doi: /10.1186/s12981-020-00322-y.
Gbadamosi SO, Itanyi IU, Menson WNA, Olawepo JO, Bruno T, Ogidi AG et al. Targeted HIV Testing for Male Partners of HIV-Positive Pregnant Women in a High Prevalence Setting in Nigeria. PLoS One. 2019; 14(1): 211022. doi: 10.1371/journal.pone.0211022.
Dionne-Odom J, Tita ATN, Silverman NS. Hepatitis B in Pregnancy: Screening, Treatment, and Prevention of Vertical Transmission. American Journal of Obstetrics and Gynecology. 2016; 214(1): 6–14. doi: 10.1016/j.ajog.2015.09.100.
Mboya E, Mizinduko M, Balandya B, Mushi J, Sabasaba A, Amani DE et al. HIV Burden and Global Fast-Track Targets Progress Among Pregnant Women in Tanzania: Analysis Of 2020 Antenatal Clinics HIV Sentinel Site Surveillance. PLoS One. 2023; 18(10): 285962. doi: 10.1371/journal.pone.0285962.
Njau AF, Robert M, Rwebembera A, Kisendi R, Maro C, Dennis G et al. Prevalence and Associated Factors for HIV, HBV, and Syphilis Co-Infections Among Pregnant Women Attending Antenatal Care in Tanzania. PLoS One. 2025; 20(8): 329068. doi: 10.1371/journal.pone.0329068.
Pereira GF, Sabidó M, Caruso A, Oliveira SB, Mesquita F, Benzaken AS. HIV Prevalence Among Pregnant Women in Brazil: A National Survey. Revista Brasileira de Ginecologia e Obstetrícia. 2016; 38(8): 391–398. doi: 10.1055/s-0036-1592102.
UNFPA Caribbean. HIV and AIDS Overview for the Caribbean region. UNFPA; 2025.
Karena ZV, Padaliya D, Viradia DS. Trend of HIV Prevalence in Antenatal Women in a Tertiary Care Hospital in the Western Region of India. Cureus. 2024; 16(3): 57125. doi: 10.7759/cureus. 57125.
Eleje GU, Onubogu CU, Fiebai PO, Mbachu II, Akaba GO, Loto OM et al. Mother-to-Child Transmission of HIV, Hepatitis B, and Hepatitis C Among Pregnant Women with Single, Dual, or Triplex Infections in Nigeria: A Systematic Review and Meta-Analysis. SAGE Open Medicine. 2022: 10. doi: 10.1177/20503121221095411.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Pakistan Journal of Health Sciences

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open-access journal and all the published articles / items are distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. For comments



