Evaluation of In-vivo anti-inflammatory activity of methyl 2-(5-butyl-6-thioxo-1, 3, 5-thiadiazinan-3yl), butanoate : In-vivo anti-inflammatory Activity of Methyl Thiadiazinan-3yl Butanoate

Usman Muhammad; Rahim Ullah; Zakia Subhan; Haji Bahadar; Sher Ahmad; Abdur Rasheed

doi:10.54393/pjhs.v5i03.1338

Authors

Usman Muhammad Institute of Pharmaceutical Sciences, Khyber Medical University, Peshawar, Pakistan
Rahim Ullah Department of Pharmacy Sarhad University of Science and Technology, Peshawar, Pakistan
Zakia Subhan Khyber Medical University, Institute of Medical Sciences, KIMS, Kohat, Pakistan
Haji Bahadar Institute of Pharmaceutical Sciences, Khyber Medical University, Peshawar, Pakistan
Sher Ahmad Department of Pharmacy, University of Peshawar, Pakistan
Abdur Rasheed Department of Pharmacy, University of Peshawar, Pakistan

DOI:

https://doi.org/10.54393/pjhs.v5i03.1338

Keywords:

Inflammation, Inflammatory Mediators, Tissue Injury, Cytokines, Oxidative Stress

Abstract

Inflammation is the body's way of fending off damage. Nonetheless, continuous and increasing inflammation may lead to the development of some chronic illnesses. It is still needed to evaluate new and safe anti-inflammatory therapies because existing medications have major side effects. Objective: To examine a novel compound's anti-inflammatory properties of methyl 2-(5-butyl-6-thioxo-1, 3, 5-thiadiazinan-3yl), butanoate (MBTTB) in albino mice models. Method: To test the anti-inflammatory effects in vivo, carrageenan-induced paw edema in albino mice of both sexes was utilized, and inflammation was measured by using Digital Plethysmometer. After extraction of carrageenan induced paw edematous, tissue level of inflammatory mediators like TNF-α, IL-1β, NF-ĸβ, and cox2 was ascertained by Western Blot analysis. Result: Based on in-vivo anti-inflammatory effects, compound MBTTB was shown to exhibit a maximum percentage inhibition of 16.7% after 1 hour, which is comparable to the standard drugs 12.8%. Following three hours, compound MBTTB showed the highest percentage inhibition of 23.2%, while the standard was 14.5%. Compound MBTTB showed the highest percentage inhibition after five hours (26.2 %), while the standard was 15.4%. The expression of many mediators' proteins, including TNF-α, IL-1β, NF-ĸβ, and COX-2, was ascertained. The computational analysis was carried out to forecast the level of decline of various mediators, including Tumor Necrosis Factor-α, (TNF) Interleukin-1β, NF-ĸβ, and COX-2. Conclusion: When considered as a whole, our current research indicates that MBTTB is a viable option for preventing inflammation in mice that has been caused by carrageenan in edematous paw.

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