Comparative Study of the Combined Antibacterial Effects of Grape Seed and Cranberry Fruit Extracts on Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli: Antibacterial Effects of Grape Seed and Cranberry Extracts

Ali Nawaz Bijarani; Ulfat Sultana; Nasima Iqbal; Nabeela Naeem; Sidra Irshad; Momina Khadija Abbasi

doi:10.54393/pjhs.v5i07.1608

Authors

Ali Nawaz Bijarani Department of Pharmacology, Shaheed Mohtarma Benazir Bhutto Medical College Lyari, Karachi, Pakistan
Ulfat Sultana Department of Pharmacology, Muhammad College of Medicine, Peshawar, Pakistan
Nasima Iqbal Department of Pathology, Baqai Medical University, Karachi, Pakistan
Nabeela Naeem Department of Pathology, Watim Medical and Dental College, Rawat, Pakistan
Sidra Irshad Department of Pharmacology, Muhammad College of Medicine, Peshawar, Pakistan
Momina Khadija Abbasi Department of Pathology, Watim Medical and Dental College, Rawat, Pakistan

DOI:

https://doi.org/10.54393/pjhs.v5i07.1608

Keywords:

Antibiotic Resistance, Pharmacodynamics, Microbial Sensitivity Test

Abstract

Antimicrobial resistance is a threat to global healthcare system. Therefore, there has been growing interest towards the natural plants which possess antimicrobial properties. Objective: The key goal of the current research is to find out the combined effect of cranberry fruit and grape seed extracts against Extended-Spectrum Beta-Lactamase (ESBL) Escherichia coli strains. Methods: E.coli bacteria with ESBL resistance were collected from the patient’s samples having bacterial infection. The extracts, grape seeds and cranberry fruits extract were prepared in different concentrations. Antibiotic susceptibility testing was done with different concentrations of the extracts, using agar dilution methods to assess their antibacterial efficacy against Meropenem and Linezolid. SPSS version 21.0 was used for data analysis. Results: The combined effect of cranberry fruit and grape seed extracts demonstrated significant antibacterial influence in counter to decrease ESBL E.coli strains. There were 40 specimens, with 45% female and 55% male, with an age range between 10 to 80 years old. Both the plant extract revealed high sensitivity against ESBL E.coli, with 95% sensitivity at the highest concentrations. The combination of CFE and GSE (50mg+60mg) showed more than 90% sensitivity, more than commonly used antibiotics like Meropenem and Linezolid. Conclusions: The combined effect of cranberry fruit and grape seed extracts showed excellent effect against antimicrobial resistance, particularly in ESBL-producing E.coli strains. The finding suggested the increase potential of natural products as alternative antimicrobial agents.

References

Hutchison C. Wars and sweets: microbes, medicines and other moderns in and beyond the (ir) antibiotic era. Medical Humanities. 2022 Sep; 48(3): 359-70. doi: 10.1136/medhum-2021-012366.

Salam MA, Al-Amin MY, Salam MT, Pawar JS, Akhter N, Rabaan AA et al. Antimicrobial resistance: a growing serious threat for global public health. InHealthcare. 2023 Jul; 11(13): 1946. doi: 10.3390/healthcare11131946.

Ahmed SK, Hussein S, Qurbani K, Ibrahim RH, Fareeq A, Mahmood KA et al. Antimicrobial resistance: impacts, challenges, and future prospects. Journal of Medicine, Surgery and Public Health. 2024 Apr; 2: 100081. doi: 10.1016/j.glmedi.2024.100081.

Endale H, Mathewos M, Abdeta D. Potential causes of spread of antimicrobial resistance and preventive measures in one health perspective-a review. Infection and Drug Resistance. 2023 Dec: 7515-45. doi: 10.2147/IDR.S428837.

Morris S and Cerceo E. Trends, epidemiology, and management of multi-drug resistant gram-negative bacterial infections in the hospitalized setting. Antibiotics. 2020 Apr; 9(4): 196. doi: 10.3390/antibiotics9040196.

Castanheira M, Simner PJ, Bradford PA. Extended-spectrum β-lactamases: an update on their characteristics, epidemiology and detection. JAC-antimicrobial resistance. 2021 Sep; 3(3): dlab092. doi: 10.1093/jacamr/dlab092.

Husna A, Rahman MM, Badruzzaman AT, Sikder MH, Islam MR, Rahman MT et al. Extended-spectrum β-lactamases (ESBL): challenges and opportunities. Biomedicines. 2023 Oct; 11(11): 2937. doi: 10.3390/biomedicines11112937.

Huemer M, Mairpady Shambat S, Brugger SD, Zinkernagel AS. Antibiotic resistance and Persistence-Implications for human health and treatment perspectives. European Molecular Biology Organization Reports. 2020 Dec; 21(12): e51034. doi: 10.15252/embr.202051034.

Bouyahya A, Omari NE, El Hachlafi N, Jemly ME, Hakkour M, Balahbib A et al. Chemical compounds of berry-derived polyphenols and their effects on gut microbiota, inflammation, and cancer. Molecules. 2022 May; 27(10): 3286. doi: 10.3390/molecules27103286.

Wang T, Zhang R, Chen Z, Cao P, Zhou Q, Wu Q. A global bibliometric and visualized analysis of bacterial biofilm eradication from 2012 to 2022. Frontiers in Microbiology. 2023 Nov; 14: 1287964. doi: 10.3389/fmicb.2023.1287964.

Kandasamy M, Nasimuddin S, Malayan J, Nithyalakshmi J, Gnanadesikan S, Chandrasekar M. A study on antibacterial effect of grape seed extracts in common clinical and drug resistant isolates. International Journal of Clinical Trials. 2016 Jul; 3(3): 165-8. doi: 10.18203/2349-3259.ijct20162799.

Daoutidou M, Plessas S, Alexopoulos A, Mantzourani I. Assessment of antimicrobial activity of pomegranate, cranberry, and black chokeberry extracts against foodborne pathogens. Foods. 2021 Feb; 10(3): 486. doi: 10.3390/foods10030486.

Tamkutė L, Gil BM, Carballido JR, Pukalskienė M, Venskutonis PR. Effect of cranberry pomace extracts isolated by pressurized ethanol and water on the inhibition of food pathogenic/spoilage bacteria and the quality of pork products. Food Research International. 2019 Jun; 120: 38-51. doi: 10.1016/j.foodres.2019.02.025.

Krasteva D, Ivanov Y, Chengolova Z, Godjevargova T. Antimicrobial potential, antioxidant activity, and phenolic content of grape seed extracts from four grape varieties. Microorganisms. 2023 Feb; 11(2): 395. doi: 10.3390/microorganisms11020395.

Helmy YA, Taha-Abdelaziz K, Hawwas HA, Ghosh S, AlKafaas SS, Moawad MM et al. Antimicrobial resistance and recent alternatives to antibiotics for the control of bacterial pathogens with an emphasis on foodborne pathogens. Antibiotics. 2023 Jan; 12(2): 274. doi: 10.3390/antibiotics12020274.

Scharf B, Schmidt TJ, Rabbani S, Stork C, Dobrindt U, Sendker J et al. Antiadhesive natural products against uropathogenic E. coli: What can we learn from cranberry extract?. Journal of Ethnopharmacology. 2020 Jul; 257: 112889. doi: 10.1016/j.jep.2020.112889.

Debalke D, Birhan M, Kinubeh A, Yayeh M. Assessments of Antibacterial Effects of Aqueous‐Ethanolic Extracts of Sida rhombifolia's Aerial Part. The Scientific World Journal. 2018 Dec; 2018(1): 8429809. doi: 10.1155/2018/8429809.

Memar MY, Adibkia K, Farajnia S, Kafil HS, Yekani M, Alizadeh N et al. The grape seed extract: a natural antimicrobial agent against different pathogens. Reviews and Research in Medical Microbiology. 2019 Jul; 30(3): 173-82. doi: 10.1097/MRM.0000000000000174.

Samarasinghe S, Reid R, Al-Bayati M. The anti-virulence effect of cranberry active compound proanthocyanins (PACs) on expression of genes in the third-generation cephalosporin-resistant Escherichia coli CTX-M-15 associated with urinary tract infection. Antimicrobial Resistance & Infection Control. 2019 Dec; 8: 1-9. doi: 10.1186/s13756-019-0637-9.

Jensen HD, Struve C, Christensen SB, Krogfelt KA. Cranberry juice and combinations of its organic acids are effective against experimental urinary tract infection. Frontiers in Microbiology. 2017 Apr; 8: 542. doi: 10.3389/fmicb.2017.00542.

Abu El-Wafa WM, Ahmed RH, Ramadan MA. Synergistic effects of pomegranate and rosemary extracts in combination with antibiotics against antibiotic resistance and biofilm formation of Pseudomonas aeruginosa. Brazilian Journal of Microbiology. 2020 Sep; 51(3): 1079-92. doi: 10.1007/s42770-020-00284-3.

Atta S, Waseem D, Fatima H, Naz I, Rasheed F, Kanwal N. Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates. Saudi Journal of Biological Sciences. 2023 Mar; 30(3): 103576. doi: 10.1016/j.sjbs.2023.103576.